INNOVATIVE 3D PRINTED NANOCOMPOSITE CONSTRUCTIONS OBTAINED FROM MARINE RESOURCES (ALGINATE, SALECAN) AND NATURAL CLAY WITH SPECIFIC APPLICATIONS IN BONE REGENERATION - 3D_ALSAC

DESCRIPTION

Finacing: State budget
Programme: PNCDI III-Program 2: Increasing the competitiveness of the Romanian economy through research, development and innovation, Demonstration experimental project (PED)
Project code: PN-III-P2-2.1-PED-2019-4216
Project title: Innovative 3D printed nanocomposite constructions obtained from marine resources (alginate, salecan) and natural clay with specific applications in bone regeneration (3D_ALSAC)
Contract No.: 332/2020
Total value: 600000 lei
State budget value: 600000 lei
Project duration: 24 months
Contract period: 03.08.2020 - 02.08.2022
Contractor: National Research & Development Institute for Chemistry and Petrochemistry - ICECHIM Bucharest
Contracting authority: The Executive Unit for Financing Higher Education, Research, Development and Innovation
Project director: PhD Raluca Ianchis; e-mail: ralumoc@yahoo.com; raluca.ianchis@icechim-pd.ro


PARTNERS

Coordinator:NATIONAL RESEARCH & DEVELOPMENT INSTITUTE FOR CHEMISTRY AND PETROCHEMISTRY-ICECHIM, 202 Spl. Independentei, Bucharest, 060021, tel: 021.315.32.99, fax: 021.312.34.93
Project manager: Dr. ing. Raluca Ianchis, tel. 021 316 30 93/int 132, email: ralumoc@yahoo.com; raluca.ianchis@icechim-pd.ro
Partner 1: "POLITEHNICA" UNIVERSITY OF BUCHAREST, 313 Spl. Independentei, 6-th sector, 60042, tel: 021 402 3927, fax: 021.402.39.35
Scientific manager: PhD Horia Iovu, tel. 0745.123.211, Fax: 021.311.17.96, email: iovu@tsocm.pub.ro
Partner 2: GENETIC LAB S.R.L., 43B Ghencea Blvd., Bucharest, 6-th sector, 012273, tel: 0747.225.341, fax: 021.440.18.78, e-mail: office@geneticlab.ro
Scientific manager: Lorand Savu, fax: 021.440.18.78, email: lorand_savu@yahoo.com


SUMMARY

The scope of 3D_ALSAC project is to develop and test demonstrative models for a new product and its associated technology, namely an efficient and improved printing formulation based on an innovative biomaterial, followed by the construction of a multifunctional 3D implant designed for tissue regeneration. Based on a solid foundation built from the theoretical and experimental results previously obtained by the partners of the Consortium, thus justifying TRL2, the objective of this project is to target TRL4, where an innovative 3D printed product based on biopolymer-clay nanocomposite will be developed, tested and validated. The concept of the 3D_ALSAC project resonates with the long-term vision of the research teams and especially that of the economic agent, of developing custom extracellular matrices for bone regeneration using the patient's stem cells. Thus, the 3D_ALSAC project opens new possibilities for collaboration with the economic environment in order to implement efficient and cost-effective solutions. The 3D_ALSAC project will lead to the evolution of the fields of materials science and regenerative medicine by repairing parts of the skeleton of the human body using innovative biomaterial based inks and 3D printing techniques and obtaining personalized implants, serving as the basis for new therapies used for bone tissue regeneration.


OBJECTIVE

The objective of 3D_ALSAC project is to develop and test demonstrative models for a new product and its associated technology, namely an efficient and improved printing formulation based on an innovative biomaterial, followed by the construction of a multifunctional 3D implant designed for bone tissue regeneration.

RESULTS

ESTIMATED

• 1 method of synthesis of the ALSA-CGF system;
• 1 optimized process for obtaining the product ALSA-CGF / 3D_ALSA-CGF;
• 1 experimental laboratory model for the product ALSA-CGF / 3D_ALSA-CGF;
• 1 optimized laboratory technology for the product ALSA-CGF / 3D_ALSA-CGF;
• 1 working protocol for the validation of the experimental laboratory model;
• 1 web page for project promotion;
• 2 patent applications;
• participation in at least 7 national/international scientific events;
• 4 articles sent for publication;
• contributions to the development of 2 doctoral/master/bachelor theses.

1. R.L. Alexa, H. Iovu, I.C. Radu, G. Vlasceanu, C.M. Ninciuleanu, E. Alexandrescu, C. Mihaescu, C. Scomoroscenco, C. Nistor, C. Petcu, R. Ianchis, "Innovative Hydrogel Based Inks with Application in Tissue Engineering", Priorities of Chemistry for a Sustainable Development - PRIOCHEM XVI ed., Bucharest, Romania, 28-30 October (2020).
2. R.L. Alexa, H. Iovu, C. Zaharia, C. Ninciuleanu, B. Trica, E. Alexandrescu, S. Preda, A. Serafim, I.C. Radu, G. Vlasceanu, L. Miclea, R. Ianchis, "Novel Biopolymeric Based Composites Inks for Tissue Engineering Application", 3rd International Conference on Emerging Technologies in Materials Engineering-EmergeMAT, Bucharest, Romania, 29-30 October (2020).

1. R.L. Alexa, H. Iovu, J. Ghitman, A. Serafim, C. Stavarache, M.M. Marin, R. Ianchis, "3D-printed gelatin methacryloyl-based scaffolds with potential application in tissue engineering", Polymers, 2021, 13(5), 727.
2. R.L. Alexa, H. Iovu, B. Trica, C. Zaharia, A. Serafim, E. Alexandrescu, I.C. Radu, G. Vlasceanu, S. Preda, C.M. Ninciuleanu, R. Ianchis, "Assessment of naturally sourced mineral clays for the 3D printing of biopolymer-based nanocomposite inks", Nanomaterials, 2021, 11(3), 703.
3. C.M. Ninciuleanu, R. Ianchis, E. Alexandrescu, C.I. Mihaescu, C. Scomoroscenco, C.L. Nistor, S. Preda, C. Petcu, M. Teodorescu, "The effects of monomer, crosslinking agent, and filler concentrations on the viscoelastic and swelling properties of poly(methacrylic acid) hydrogels: A Comparison", Materials, 2021, 14(91), 2305.
4. R. L. Alexa, H. Iovu, M.C. Nicolae, I.C. Mihaescu, E. Alexandrescu, R.Ianchis, "3D printing of super concentrated alginate clay ink with potential application in regenerative medicine", UPB Scientific Bulletin, Series B: Chemistry and Materials, 2021.
5. R.L Alexa, R. Ianchis, D. Savu, M. Temelie, B. Trica, A. Serafim, G.M. Vlasceanu, E. Alexandrescu, S. Preda, H. Iovu, "3D Printing of Alginate-Natural Clay Hydrogel-Based Nanocomposites", Gels, 2021, 7(4), 211.

1. R.L. Alexa, H. Iovu, G.Vlasceanu, A. Serafim, E. Alexandrescu, S. Preda, R. Ianchis, "3D Printable ink based on alginate and layered silicates", NeXT-Chem III, Bucharest, Romania (2021).
2. C.M. Ninciuleanu, R. Ianchis, E. Alexandrescu, I.C. Mihaescu, S. Preda, C. Scomoroscenco, S. Burlacu, L.C. Nistor, C. Petcu, M. Teodorescu, "Polymetchacrylic Acid Composite Hydrogels with Potential Uses As Devices in Medical and Environmental Protection Fields", Webinar on Nanotechnology-Phronesis LLC, 25-26 January (2021).
3. R.L. Alexa, H. Iovu, G. Vlasceanu, A. Serafim, E. Alexandrescu, S. Preda, R. Ianchis, "Scaffolds obtained by 3D Printing based on alginate-clay hybrid nanocomposites", Bucharest Polymer Conference 2-nd Edition, University POLITEHNICA of Bucharest, Romania, 10-11 June (2021).
4. R. Ianchis, C.M. Ninciuleanu, E. Alexandrescu, I.C. Gifu, R. Gabor, C. Mihaescu, C. Scomoroscenco, S. Nitu, C. Nistor, C. Petcu, H. Iovu, R.L. Alexa, "Investigation of crosslinked hydrogels based on Salecan biopolymer", Bucharest Polymer Conference 2-nd Edition, University POLITEHNICA of Bucharest, Romania, 10-11 June (2021).
5. M.M. Marin, R.Leu, M.G. Albu Kaya, E. Alexandrescu, S. Preda, H. Iovu, R. Ianchis, "Development of New Collagen/Clay Composite Biomaterials", Bucharest Polymer Conference 2-nd Edition, University POLITEHNICA of Bucharest, Romania, 10-11 June (2021).
6. R.L. Alexa, I.C. Gifu, C. Ninciuleanu, E. Alexandrescu, C. Scomoroscenco, C. Mihaescu, S. Burlacu, C.L. Nistor, C. Petcu, H. Iovu, R. Ianchis, "3D printing of novel polysaccharide based biomaterials foreseen for biomedical applications", 4^th International Conference on Emerging Technologies in Materials Engineering-EmergeMAT, Bucharest, Romania, 4-5 November (2021).
7. R.L. Alexa, C. Ninciuleanu, E. Alexandrescu, C. Mihaescu, S. Preda, C.L. Nistor, C. Petcu, H. Iovu, L. Savu, R. Ianchis, "Synthesis of platelet rich plasma enriched inorganic advanced material with application in regenerative medicine", 4^th International Conference on Emerging Technologies in Materials Engineering-EmergeMAT, Bucharest, Romania, 4-5 November (2021).
8. M.M. Marin, I.C. Gifu, C. Ninciuleanu, E. Alexandrescu, C. Scomoroscenco, S. Burlacu,C.L. Nistor, C. Petcu, H. Iovu, R.L. Alexa, R. Ianchis, "Synthesis and characterization of green crosslinked hydrogels", 4^th International Conference on Emerging Technologies in Materials Engineering-EmergeMAT, Bucharest, Romania, 4-5 November (2021).

1. R. Ianchis, R.L. Alexa, M.M. Marin, C.I. Gifu, C.M. Ninciuleanu, E. Alexandrescu, C. Scomoroscenco, S.G. Burlacu, C.I. Mihaescu, C.L. Nistor, C. Petcu, H. Iovu, "Composition and process for obtaining hydrogels based on salecan and their use for additive manufacturing", A 2021-00643/25.10.2021

1. M.M. Marin, R.Ianchis, R.L. Alexa, I.C. Gifu, M.G.A. Kaya, D.I. Savu, R.C. Popescu, E. Alexandrescu, C.M. Ninciuleanu, S. Preda, M. Ignat, R. Constantinescu, H. Iovu, "Development of New Collagen/Clay Composite Biomaterials", Int. J. Mol. Sci., 2022, 23 (10), 401.
2. R.L. Alexa, A. Cucuruz, C.D. Ghitulica, G. Voicu, L.R. Stamat (Balahura), S. Dinescu, G.M. Vlasceanu, C. Stavarache, R. Ianchis, H. Iovu, M. Costache, "3D printable composite biomaterials based on GelMA and hydroxyapatite powders doped with cerium ions for bone tissue regeneration", Int. J. Mol. Sci., 2022, 23(3), 1841.

1. R. Ianchis, I.C. Gifu, E. Alexandrescu, M.M. Marin, S. Burlacu, C. Mihaescu, C.L. Nistor, C. Petcu, "The synthesis of novel polyssacharide based nanocomposite materials. The study of inorganic filler concentration", Virtual Conference on Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2022 – Summer Edition, 22-24 June (2022).
2. R. Ianchis, I.C. Gifu, E. Alexandrescu, M.M. Marin, S. Burlacu, C. Mihaescu, C.L. Nistor, C. Petcu, "The synthesis of novel polyssacharide based nanocomposite materials. Comparative study of Cloisite clay type", Virtual Conference on Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2022 – Summer Edition, 22-24 June (2022).

1. R. Ianchis, M.M. Marin, R.L. Alexa, C.I. Gifu, C.M. Ninciuleanu, E. Alexandrescu, C. Scomoroscenco, S.G. Burlacu, C.I. Mihaescu, C.L. Nistor, C. Petcu, H. Iovu, "Compositions and process for obtaining composite hydrogels based on natural polysaccharides and their application in the three-dimensional printing process", A 2022-00127/16.03.2022